Dr. Kiran D. Rasal
Scientist(Sr. Scale)
Fish Genetics and Biotechnology
Fish Genetics and Biotechnology
MFSc. / Ph. D.
+919820588367 /+917978481220
Research Interests
- Revelation of genes associated with reproduction and metabolism in fish
- Functional genomics in aquaculture
- Fish Transgenics & Genome editing using CRISPR/Cas9
Research Papers
- Pokanti Vinay Kumar; Kiran D. Rasal; Arpit Acharya; et al. (2023) Muscle transcriptome sequencing revealed thermal stress responsive regulatory genes in farmed rohu, Labeo rohita (Hamilton, 1822). Marine Biotechnology, (https://doi.org/10.1007/s10126-023-10259-8)
- Kiran D. Rasal; Sujata Mohapatra; Pokanti et al. (2023) DNA Methylation Profiling of Ovarian Tissue of Climbing Perch (Anabas testudienus) in Response to Monocrotophos Exposure. Marine Biotechnology, (https://doi.org/10.1007/s10126-023-10264-x).
- Prachi Asgolkar; Kiran D. Rasal*; et al. (2023) Molecular Characterization of Gonadotropin Releasing Hormone Receptor and its Expression Profiling in Response to HCG Stimulation in Striped Murrel, Channa striata (Bloch, 1793). Gene Reports, Elseiver. (https://doi.org/10.1016/j.genrep.2023.101850).
- Raju Ram, A P-Kumar, Chandan Haldar, Gireesh-Babu Pathakota, Kiran Rasal (2023) Molecular cloning and expression profiling of insulinlike growth factor 2 and IGF-binding protein 6 in Clarias magur (Hamilton 1822), Animal Biotechnology, 34:7, 2262-2272, DOI: 10.1080/10495398.2022.2086561
- Yashwanth, B.S., Pinto, N., Sathiyanarayanan, A. Kiran Rasal et al. (2023). Functional characterization of Labeo rohita muscle cell line for in vitro research. Mol Biol Rep 50, 5635–5646 https://doi.org/10.1007/s11033-023-08427-z
- Sarika Jaiswal, Kiran D. Rasal, Tilak Chandra, Ratna Prabha, Mir Asif Iquebal, et al, (2023) Proteomics in fish health and aquaculture productivity management: Status and future perspectives, Aquaculture, 566, https://doi.org/10.1016/j.aquaculture.2022.739159.
- Siddaiah, G. M., Kumar, R., Kumari, R., Damle, D. K., Rasal, K. D., et al. (2022). Dietary supplementation of fish protein hydrolysate improves growth, feed efficiency and immune response in freshwater carnivore fish, Channa striata fingerlings. Aqua. Research, 53, 3401–3415. https://doi.org/10.1111/are.15848
- Jitendra Kumar Sundaray, Sangita Dixit, Ashraf Rather, Kiran D. Rasal, Lakshman Sahoo, (2022) Aquaculture omics: An update on the current status of research and data analysis, Marine Genomics, 64, https://doi.org/10.1016/j.margen.2022.100967.
- Patra, K., Rajaswini, R., Murmu, B. Kiran Rasal et al. (2022) Identifying miRNAs in the modulation of gene regulation associated with ammonia toxicity in catfish, Clarias magur (Linnaeus, 1758). Mol Biol Rep 49, 6249–6259 (doi.org/10.1007/s11033-022-07424-y).
- Rasal, K.D. and Sundaray, J.K. (2020), Status of genetic and genomic approaches for delineating biological information and improving aquaculture production of farmed rohu, Labeo rohita (Ham, 1822). Review Aquaculture, https://doi.org/10.1111/raq.12444
- Rasal, K.D., M.A.Iquebal, A.Pandey, P. Behera, S.Jaiswal, M. Vasam, S. Dixit, M. Raza, L. Sahoo, S. Nandi, UB Angadi, A. Rai, N. Nagpure, A. Chaudhari, D. Kumar, and J.K. Sundaray (2019) Revealing liver specific microRNAs linked with carbohydrate metabolism of farmed carp, Labeo rohita (Hamilton, 1822), Genomics doi.org/10.1016/j.ygeno.2019.07.010
- L. Sahoo, S. Sahoo, M. Mohanty, M. Sankar, S. Dixit, P. Das, Rasal, K. D, M. A. Rather & J. K. Sundaray (2019) Molecular characterization, computational analysis and expression profiling of Dmrt1 gene in Indian major carp, Labeo rohita (Hamilton 1822), Animal Biotechnology, DOI: 10.1080/10495398.2019.170768
- Rasal, K.D., Iquebal, M.A., Jaiswal, S. et al. (2019). Liver-specific microRNA identification in farmed carp, Labeo bata (Hamilton, 1822), fed with starch diet using high-throughput sequencing. Mar Biotechnol https://doi.org/10.1007/s1126-019-09912-y
- Barman, H.K., Rasal, K.D., Mondal S., (2019) Status and prospects of gene editing and transgenic in fishes, Indian J. Genet., 79(1) 10.31742/IJGPB.79S.1.21 .
- Rasal, K. D., Nandanpawar, Priyanka C. Swain, Pranati Badhe, Mohan R. Sundaray, Jitendra Kumar Jayasankar, Pallipuram. (2016) MicroRNA in aquaculture fishes: a way forward with high-throughput sequencing and a computational approach, Reviews in Fish Biology and Fisheries,. 10.1007/s11160-016-9421-6
- Rasal, K.D., Vemulawada C, Swagat Kumar P, Sasmita J, Shibani Dutta M, Swapnarani N, Jitendra Kumar S, Pallipuram J, Hirak Kumar B, (2016) Identification of deleterious mutations in Myostatin (MSTN) gene of rohu carp (Labeo rohita) using modelling and molecular dynamic simulation approaches. Biomed Res International (Article ID 619169) DOI:10.1155/2016/7562368
- Chakrapani V., Patra S. K., Mohapatra S.D., Rasal, K.D., U. Deshpande, Nayak S, Jitendra K. Sundaray, P. Jayasankar, H. K. Barman: Comparative transcriptomic profiling of larvae and post-larvae of Macrobrachiumrosenbergii in response to metamorphosis and salinity exposure. Genes and Genomics 07/2016; 38(11)., DOI:10.1007/s13258-016-0452-0.
- Vemulawada Chakrapani, Rasal, K.D., Shibani D. Mohapatra, Jitendra K. Sundaray, Pallipuram Jayasankar and Hirak K. Barman. (2018) In silico analysis of nsSNPs of carp TLR22 gene affecting its binding ability with poly I:C, Interdisciplinary Sciences: Computational Life Sciences, 10, 641–652, 10.1007/s12539-017-0247-2
- V Chakrapani, Rasal, K.D., SD Mohapatra, AR Rasal, P Jayasankar, Barman HK, (2017) Molecular characterization, computational analysis and transcript profiling of glutamate dehydrogenase ( gdh ) gene of Macrobrachium rosenbergii exposed to saline water, Gene Reports, 8, 37-44
- Pawar N, Gireesh-Babu P, Sabnis S, Rasal K. D., Murthy R, Zaidi SG, Sivasubbu S, Chaudhari A. (2016) Development of a fluorescent transgenic zebrafish biosensor for sensing aquatic heavy metal pollution. Transgenic Res. PMID: 27120052
Books/Manual Edited
- Rasal K.D., Rasal, A. R., Patil, P.A., Kamble, S.P., Mohapatra, B.C., & Sundaray, J.K., (2016) Carp Mashanche Godya Panyatil Matsyapalan. CIFA Publications, ISSN No 978-81-924918-6-8
- Compiled and edited Summer School Training Manual on “Advance tools for genetic improvement of aquaculture species: an Integrated Approach, during 28 – 30 January, held at Central Institute of Freshwater Aquaculture, Odisha.
- Compiled & edited Summer workshop Manual “Bioinformatics tools for fish Genomics”, during 21st-22nd 2015 at ICAR-CIFA, Bhubaneswar.
- Training Manual Hands on Training on Analysis of Biological Data using Computational Tools” on 5-14th July, 2016 at ICAR-CIFA, Bhubaneswar.
- Training Manual tribal farmers of Pune region regarding Freshwater Aquaculture during 25-28 March, 2017 at ICAR-CIFA, Bhubaneswar.
- Compiled and edited Abstract book of 3rd International Symposium on Genomics in Aquaculture, during 21 – 23 January, 2020 held at ICAR-Central Institute of Freshwater Aquaculture, Odisha
- Compiled and edited Souvenir book of 3rd International Symposium on Genomics in Aquaculture, during 21 – 23 January, 2020 held at ICAR-Central Institute of Freshwater Aquaculture, Odisha
Extension leaflets
- Patil, P.A., Kamble, S.P., Rasal, K.D., Rasal, A.B., Mahapatra, B.C., Sundaray, J.K. and Shikare, V.B., 2016. Carp Masliche Matsyasawardhan. pp.1-4. Publisher: ICAR- CIFA. Ext. Series No. 18. (Marathi Edition Leaflet: IMC fish culture).
- Rasal, K.D., Patil, P.A., Rasal, A.B., Mahapatra, B.C. and Sundaray, J.K., 2016. Portable FRP Carp Masali Hatchery. pp.1-4. Publisher: ICAR- CIFA. Ext. Series No. 19. (Marathi Edition Leaflet: Portable FRP Carp Fish Hatchery).
Institute Funded Project
| Sr. No | Project Title | Role in Project | Duration |
|---|---|---|---|
| 1. | Identification of epigenetic markers associated with growth performance in Clarias magur (Hamilton, 1822) | PI | April 2021– March 2024 |
| 2. | Evaluation of RNA-guided recombinase (RGR) platform for cell-independent and safer genome engineering in zebrafish vertebrate model | Co-PI | May 2022 – April 2025 |
| 3. | Utilization of Jojoba (Simmondsia chinensis) and Mahua (Madhua indica) Cake / Meal-based Products in Aquafeed | Co-PI | May 2022 – April 2025 |
| 4. | In-vitro production of fertile sperms from the testicular cells of Clarias batrachus (Project:I-59/m) | Co-PI | April 2012 – March 2015 |
| 5. | Implication of fat accumulation on gonadal maturation in Catla catla. (PI:1-59/K) | Co-PI |
May 2019 – April 2021 (Till July 2020) |
Externally Funded Projects
| Sr. No | Project Title | Role in Project | Funding Agency | Duration |
|---|---|---|---|---|
| 1. | Applications of CRISPR/Cas system in Molecular Detection of Fish and Shrimp Diseases (Department of Biotechnology (DBT), GoI) | Co-PI | -- | April 2022 – March 2025 |
| 2. | Identification and comparative expression analysis of novel immune-related genes against prevalent bacterial infections and development of remedial messures in Asian Seabass, Lates calcarifer (Department of Biotechnology (DBT)) | Co-PI | -- | May 2023 – April 2025 |
| 3. | CRP Genomics Platform _ Whole Genome Sequencing of Labeo fimbriatus (ICAR Network project) | Co-PI | -- | April 2023-March 2025 |
| 4. | Captive breeding of hilsa, Tenualosa ilisha :Phase II (ICAR NASF project) | Co-PI | -- | April 2023- March 2024 |
| 5. | Molecular and computational approach to delineate metabolic pathways for better carbohydrate utilization in Labeo species(PI: Dr. J. K. Sundaray) (Budget-45 Lakhs) | Co-PI | ICAR-CABin Network Project | March 2015- April 2017 |
| 6. | Centre of Excellence on Genetic enhancement of economically important freshwater fish, Catla catla using genetic and molecular approaches (PI: Dr. J. K. Sundaray) (Budget- 435 Lakhs) | Co-PI | DBT |
Dec 2018 - Dec 2020 (Till July 2020) |
| 7. | Deciphering genetic variation in the carbohydrate metabolism of farmed rohu families. (PI: Dr. J. K. Sundaray) (Budget-60 Lakhs) | Co-PI | ICAR-CABin Network Project | March 2017-April 2020 |
| 8. | Value addition by generating stable lines of ornamental transgenic freshwater fishes (PI: Dr. H.K. Barman) (Budget-62 Lakhs) | Co-PI | DBT | June 2017 -May 2020 |
Awards/Prizes/Scholarships Received
- Awarded as Best Scientist in ICAR-CIFA, Bhubaneswar for the year 2015-16.
- Felicitated as a Young Scientist Award with medal during Young Scientist Meet-2016 organized by ZSI, Kolkata during 4-6 Feb, 2016.
- Received Best poster award: S. Nayak, S. Ferosekhan, S.K. Sahoo, L. Patnaik, J.K. Sundaray, Rasal K.D., P. Jayasankar, H.K. Barman (2016) In vitro production of fertile sperm from the cultivated spermatogonial stem cells of Clarias batrachus. 2nd International Symposium on Genomics in Aquaculture, during 28 – 30 January, 2016 held at Central Institute of Freshwater Aquaculture, Odisha.
- Received Best poster award; Rasal K.D. and J.K. Sundaray (2019), “Regulatory role of microRNAs in the fish nutritional physiology and reproductive biology” 6th International Conference on Fisheries and Aquaculture 2019 – (ICFA 2019) held in Bangkok, Thailand during 23-25 Aug, 2019.
- Received Best oral presentation award: Rasal K.D. and J.K. Sundaray (2020) “Integrated microRNA and mRNA analysis reveal regulatory pathways underlying the carbohydrate metabolism in farmed rohu, Labeo rohita” in 3rd International Symposium on Genomics in Aquaculture, during 21 – 23 January, 2020 held at ICAR-Central Institute of Freshwater Aquaculture, Odisha
Database Development
- Muscle and liver Transcriptome data generation in Labeo rohita (NCBI Accession number: SRR8816557, SRR8816556, SRR8816555)
- Liver specific miRNAs database generation in rohu, L .rohita and L. bata (NCBI Accession number: SRR8813884, SRR8813883 and SRR8813882, 4SRR8862885, SRR8862885, SRR8862886 )
- Skin-mucus and gut microbiome data of murrel, Channa striata (NCBI Accession number: SRR10662778; SRR10662777; SRR10662776; SRR10662775)
Capacity Building Training Attended
- Attended three months professional training programme on ‘Next generation sequencing technologies for genome analysis using Bioinformatics tools ’ at Ome Research Facility, Department of Animal Biotechnology, AAU, Anand, Gujarat during 8th May-11th August 2014.
- Participated in Training program on “NGS Data Analysis organized by ICAR-CIFA from 20th Jan to 25th Jan, 2015.
- Attended capacity building CAFT training programme on “Advanced Tools for Analysis of Phenomic and Genomic Data" during 5th-25th March 2015 at ICAR-NDRI, Karnal.
- Attended Three months, DBT sponsored training on Molecular Biology and Biotechnology during1st Nov 2015 - 31st Jan 2016 at ICAR-CIFE, Mumbai.
- Attended “International symposium cum training on ‘Genome Editing Technologies and their Applications in Biology, Medicine and Agriculture’ at KIIT Bhubaneswar, India during 16-25 Feb 2017.