NAIP Research Projects
Project Title : A Value Chain On Fish Production In Fragile Agricultural Lands And
Unutilized Aquatic Resources In Maharashtra
Personnel:A.K.Reddy, V.K. Tiwari, S. Munilkumar, A. Vennila, S.P. Shukla, A.K.Verma, Ananthan, P.S.
8 nursery ponds were stocked with carp (Catla and Rohu) spawn in the 3 week of August, 2009 at Shere and Gondi villages. A total of 20 lakhs spawn was stocked in 8 Nos. of nursery ponds. 2.5 lakhs carp seed was distributed to the farmers and 2.5 lakhs of fry was stocked in two grow out ponds for rising of yearlings for stocking in grow out ponds at demonstration sites and supply to demonstration farmers. 3 grow out ponds at Shere village were stocked with carp yearlings of 25-40 g weight in the months of June/July, 2009. The yearlings were procured from a private party and transported for a distance of 350 km in open plastic containers of 1000 l capacity each by adding clove oil as sedative. The seed was successfully transported with 92-96 % survival. The carps have grown to 600 to 1200 g in 8-9 months. The fish which have grown to morethan 1.0 kg had been segregated and stocked separately for maintenance of brood stock. Some fishes had been sold out in live condition at farm site. In order to check the growth and survival of fingerlings of same season, two grow out ponds at Gondi were stocked with carp fingerlings of 5-7 cm size in the month of October/November, 2009. The growth of fish ranged between 300 and 700 g. The partnering NGO Vatsalya Mandir, Lanja initiated rearing of Rohu and Catla spawn up to fingerling stage and stockings were done in three reservoirs. The other partner College of Fisheries had constructed 10 Nos. of rearing ponds in the vicinity of reservoirs for rising advanced fingerlings / yearlings. The College of Fisheries, Ratnagiri formulated two fish products i.e. Fish Cutlet and Fish Shev prepared from freshwater fishes. Products were accepted fairly by the consumers. Further studies are under progress to commercialize the products. Three training programmes were organized by College of Fisheries and Vatslya Mandir on 'Preparation of value added fish Products from fresh water fishes'. In order to know the consumer preference of the two products the Vatslya Mandir participated in Food Festival organized by KVK at Lanja, where 625 consumers checked the taste of the products. Positive response was received from >90 % of the consumers. Another two products i.e. fish wafers and fish fingers were also prepared and women were trained in product preparation.
Project Title:Livelihood And Nutritional Security Of Tribal Dominated Areas Through Integrated Farming Systems And Technology Model
Personnel :R. S. Biradar, V.K. Tiwari, A. K. Reddy, M.L. Ojha (MPUAT, Udaipur)
Two brood stock ponds of 0.4 ha each were constructed. One reservoir pond of 0.1 ha constructed. Tube well with yield of 300 l/minute constructed. Loharia and Marella tanks of 5 ha each in Daiyana and Vajakaria villages respectively in Sagwadia cluster have been taken on lease for demonstration of aquaculture technologies in project area. 20 lakh spawn of IMC were raised in project nursery ponds at KVK Banswara and about 5.4 lakh fry had been harvested and stocked in farmers ponds. Site for community pond has been identified in Nokla village. Construction of hatchery shed completed and construction of hatchery proper is in progress. Two short term training programmes on 'Fish Processing and Value addition' were conducted during 18-19 January and 20-21 January,2010 for 11 and 19 participants respectively of NAIP adopted villages. As part of the training, fish pickle of about 30 kg was prepared and put into 300 packets of about 100 g each. These pickle pockets were displayed and distributed during RPC meeting held at Udaipur for assessing the taste and popularization. About 4,000 fingerlings were stocked in recently constructed stocking pond at KVK, Banswara. Rearing of carp seed and also rearing and breeding of ornamental fishes was in progress at KVK, Banswara.
Project title:Bio-prospecting Of Genes And Allele Mining For Abiotic Stress Tolerance
Personnel:A. Chaudhari, Gireesh Babu P, G. Tripathi
The major goal of the project is to characterize the four novel partial gene sequences found to be involved in salt homeostasis of P. monodon, marine tiger shrimp in an earlier study. Shrimp specimens (20g) were procured from Pancham Shrimp Farm, Saphale, Thane and acclimatized to various salinities (5, 10, 15, 25 and 35 ppt). The shrimp were maintained at final salinities for 20 days and then dissected to collect gill and muscle tissues, which were stored in RNA later and kept at -80o C until RNA extraction. Total RNA was extracted by the Triazol method and single strand cDNA prepared using standard protocol. Real time PCR primers were designed and procured for the four novel genes and other reference genes like Na+ K+ ATPase, Carbonic anhydrase and Arginine kinase. The PCR conditions were standardized, amplicons were cloned and sequenced. Full length Na+ K+ ATPase gene was amplified and cloned in pET28a His-Tag vector to overexpress in E.coli. A 108 kD protein was observed in SDPAGE after 24h induction with IPTG. Western blotting with His-tag antibody was performed to confirm the protein. No significant morphological differences were observed in either tissue in relation to salinity.
Project title:Gene Silencing- A Strategy For Management Of White Spot Syndrome Virus (WSSV)
Personnel:K.V. Rajendran, M. Makesh
The medium which supports the attachment and proliferation of cells derived from various organs of mud crab was optimized. Leibovitz L-15 media (1 X, 2 X and 3 X) and serum-free medium with antibiotics, penicillin (100units/ml), streptomycin (100 μg/ml) and amphotericin B (0.25 μg/ml) were used for culturing the cells. Growth medium was prepared by adding 5%, 10% and 20% fetal bovine serum along with 5% crab hemolymph serum. Osmolality was adjusted with 10% 2.5X crab saline or 1:1 1X crab saline to L-15. Osmolality of different media, supplements, crab saline and hemolymph was determined. Different tissues of crab such as heart, hepatopancreas, muscle, ovary, eggs, testis, hematopoeitic tissue, nerve tissue and hemocytes were used for primary culture. Testis, ovary, hepatopancreas and heart tissues were trypsinised using 0.125 & 0.25% trypsin to get single cells.
Project title:Vision Policy and Gender
Personnel:R. S. Biradar, Swadesh Prakash
The technologies which were identified for impact assessment are (a) Giant Freshwater prawn seed production using artificial seawater (b) Polyculture of carps with Macrobrachium rosenbergii (c) Carotenoid rich feed for ornamental fish culture and breeding. Interview Schedule for impact assessment of technologies had been finalized. Data analysis and report writing is in progress.
Project title:Strengthening Of Digital Library And Information Management (egranth)
List of in-house publications of CIFE was prepared. Classification of Books was undertaken. Cataloguing of books was done in connection and attempts were made to export data to www.psw.oclc.org for uploading of the prepared records.